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Team:Caltech/Week 9
From 2011.igem.org
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Transformation of I732019, I732005, J23119 from source plate in XL-10 gold.<br/> | Transformation of I732019, I732005, J23119 from source plate in XL-10 gold.<br/> | ||
Re-assembly of DDT gene using alternate method<br/> | Re-assembly of DDT gene using alternate method<br/> | ||
| + | GCMS of p450-degraded BPA | ||
===Results=== | ===Results=== | ||
| - | + | Bacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well. | |
<gallery> | <gallery> | ||
File:Plate,_show_control.jpg|96-well plate with XL-10 biofilm grown for two days, visualized with 1% crystal violet | File:Plate,_show_control.jpg|96-well plate with XL-10 biofilm grown for two days, visualized with 1% crystal violet | ||
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</gallery> | </gallery> | ||
Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands.<br/> | Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands.<br/> | ||
| + | BPA sample was too diluted for GCMS; must concentrate first. | ||
| + | ==August 9== | ||
}} | }} | ||
Revision as of 19:51, 9 August 2011
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Project |
August 8Testing for biofilms grown on 96-well plate and Erlenmeyer flasks with crystal violet ResultsBacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well.
Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands. August 9
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