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Team:TU-Delft/Notebooks/Protocols/LBMedium
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==='''LB Medium 1 liter'''=== | ==='''LB Medium 1 liter'''=== | ||
| - | 1. Measure 950 ml of demi water and put it in a | + | 1. Measure 950 ml of demi water and put it in a flas |
| + | |||
2. Add 10 grams of NaCl | 2. Add 10 grams of NaCl | ||
| + | |||
3. Add 10 grams of Bacter Tryptone | 3. Add 10 grams of Bacter Tryptone | ||
| + | |||
4. Add 5 grams of Yeast extract | 4. Add 5 grams of Yeast extract | ||
| + | |||
5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH | 5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH | ||
| + | |||
6. Fill up to 1 liter | 6. Fill up to 1 liter | ||
| + | |||
7. Bring the flask to the autoclave | 7. Bring the flask to the autoclave | ||
Revision as of 13:06, 8 August 2011
_NOTOC__
Protocol
LB Medium 1 liter
1. Measure 950 ml of demi water and put it in a flas
2. Add 10 grams of NaCl
3. Add 10 grams of Bacter Tryptone
4. Add 5 grams of Yeast extract
5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH
6. Fill up to 1 liter
7. Bring the flask to the autoclave
