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Team:Paris Liliane Bettencourt/Notebook/2011/08/06/
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(Created page with "== Cyrille == The ligation has given 7 transformant. 2 on the 3-5 200µL plate, 4 on the 6789 200µL plate and one on the 10-11 200µL plate 7 miniprep were prepared from these...")
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(Created page with "== Cyrille == The ligation has given 7 transformant. 2 on the 3-5 200µL plate, 4 on the 6789 200µL plate and one on the 10-11 200µL plate 7 miniprep were prepared from these...")
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Revision as of 15:27, 6 August 2011
Cyrille
The ligation has given 7 transformant. 2 on the 3-5 200µL plate, 4 on the 6789 200µL plate and one on the 10-11 200µL plate
7 miniprep were prepared from these clones.
Digestion:
The obtained plasmids are digested by both EcoRI and MscI from NEB in 10x NEBuffer 4.
The mix contain:
- 1µL 10x NEBuffer 4
- 2µL plasmid
- 1µL MscI
- 1µL EcoRI
- 5µL H2O
