"
Team:DTU-Denmark-2/Notebook/Lab notes
From 2011.igem.org
(→July) |
(→Thursday 21.07.2011) |
||
| Line 176: | Line 176: | ||
===Thursday 21.07.2011=== | ===Thursday 21.07.2011=== | ||
| + | |||
| + | Some more PCR! | ||
| + | |||
| + | Some of the PCR reactions that didn't work Tuesday was repeated with a different annealing temperature and amplification of new biobricks. | ||
| + | |||
| + | '''New biobricks in the bag:''' | ||
| + | |||
| + | *PGK | ||
| + | *BGHpA | ||
| + | *eGFP+k_GOI | ||
=='''August'''== | =='''August'''== | ||
Revision as of 12:25, 5 August 2011
| Home | The Team | The Project | Safety | Notebook |
Contents |
Lab notes
June
July
Tuesday 19.07.2011
First day in the lab and 48 biobricks to go, how exciting!
Of the 48 biobricks we will have when the project is finished we had only established 17 of them and ordered primers. We started the day with 11 PCR reactions of the first bash of 17 biobricks, we still need some DNA templates to due all 17. 6 PCR were correct and was purified with a purification Kit (GE Healthcare). So only 42 biobricks to go!
Biobricks in the bag:
- pALC
- TtrpC
- Terminator 1
- Terminator 2
- Terminator 3
- argB
See protocol for PCR mix and program used.
- Insert GEL Photo
Thursday 21.07.2011
Some more PCR!
Some of the PCR reactions that didn't work Tuesday was repeated with a different annealing temperature and amplification of new biobricks.
New biobricks in the bag:
- PGK
- BGHpA
- eGFP+k_GOI
August
Thursday 04.08.2011
Today we purified 22 biobricks, which have been obtained over the last week of laboratory work with PCR. The purification of DNA was done by using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) according to manufactures manual for purification of DNA from TAE and TBE agarose gels protocol, see protocol section for more information.
- HeLa cells U2OS
- pyrG TEMP
September
October
