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Team:Caltech/Week 8
From 2011.igem.org
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==August 3== | ==August 3== | ||
Colony PCR of Gibson transforms<br/> | Colony PCR of Gibson transforms<br/> | ||
| + | Emzo's protocol for traditional assembly of PCR'd pNT002 insert and pSB3K3 plasmid<br/> | ||
===Results=== | ===Results=== | ||
<table border="1"> | <table border="1"> | ||
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File:8-4colonypcr003.jpg|upper comb lane 1 NEB 2-log ladder, 2-13 pNT003 colonies 1-12 vector amplification; lower comb 1 NEB 2-log ladder, 2-13 pNT003 colonies 1-12 insert amplification | File:8-4colonypcr003.jpg|upper comb lane 1 NEB 2-log ladder, 2-13 pNT003 colonies 1-12 vector amplification; lower comb 1 NEB 2-log ladder, 2-13 pNT003 colonies 1-12 insert amplification | ||
</gallery> | </gallery> | ||
| + | No growth from traditional assembly on self ligation control or experimental plate<br/> | ||
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Revision as of 21:33, 4 August 2011
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Project |
July 31Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team ResultsMass spectrometry did not run correctly; samples did not ionize; must rerun August 1PCR pSB3K3 and pNT003 insert with the new primers. ResultsNo colonies visible on the pUC19-transformed cells The enrichment culture plates show no visible growth yet. Gel Extractions of July 29 PCR of pNT002 and pNT003 inserts
August 2Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3 Results
pNT002 ligation showed band on gel
August 3Colony PCR of Gibson transforms Results
August 4Ran gel of colony pcr from yesterday ResultsSome of the colony PCR have insert amplifications of the correct length. Will send some off for sequencing tomorrow.
No growth from traditional assembly on self ligation control or experimental plate
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