Team:Paris Liliane Bettencourt/Notebook/2011/07/18/

From 2011.igem.org

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[[File:T7 electro 20072011.jpeg|thumb|center| ladder - non digested - 0 - 1 - 3 - 5 - 6 - 7 - 8 - 9 - 10 - 11 - 13 - 15 - ladder ]]
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Revision as of 14:00, 4 August 2011

Edward

Minipreps

  • on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
  • on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
  • on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL


    Restriction Digest of RBS-spoVg and T7 RNA polymerase

    • BBa_K145001 cut by XbaI and PstI
    • BBa_K143021 cut by SpeI and PstI


      Final volume : 20 µL

      </p><p style="display:block;">DNA Gel electrophoresis (T7 RNA polymerase) </p><p style="display:block;">Protocol (6 wells) </p>

      • 1kb ladder
      • T7 undigested plasmid
      • Digested T7 (BBa_K145001 cut by XbaI and PstI)
      ladder - non digested - 0 - 1 - 3 - 5 - 6 - 7 - 8 - 9 - 10 - 11 - 13 - 15 - ladder

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