Team:Freiburg/Notebook/2 August

From 2011.igem.org

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Add 1 μl Loading dye buffer and load the gel.
Add 1 μl Loading dye buffer and load the gel.
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[[File:Freiburg 11 8 2 2011 5 41 23 PM.jpg|400px]]
 
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we dont see the expected edges.
 
===DNA Gel===
===DNA Gel===

Revision as of 11:27, 3 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

transformation with quickchanged CcaS

Investigators:Julia

Testdigest

Investigators: Jakob, Julia


Name:

Jakob

Date:

02.08.2011

Continue from Experiment 02.08.2011

Miniprep

Project Name: green light receptor, testdigest of CcaR with BamHI

For one reaction you need: For Mastermix: Number of samples+2extra


4μl H2O 20
1μl Buffer, NEB4 5
1μl BSA (10x) 5
0,5 μl Enzym 1 1 BamHI
0,5 μl Enzym 2 -
3 μl DNA 3

10 μl total volume


Give 3 μl of DNA in an eppi and add 7μl of the mastermix.

Incubate for about 1h at 37°C.


Add 1 μl Loading dye buffer and load the gel.

DNA Gel

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

transformation with Ligationreaction(pcyA+terminator) from 1st of august and the pJT122

Investigators:Julia

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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