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Copenhagen/3 August 2011
From 2011.igem.org
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[http://www.neb.com/nebecomm/products/productE5500.asp NEB User Cloning Kit] | [http://www.neb.com/nebecomm/products/productE5500.asp NEB User Cloning Kit] | ||
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Revision as of 10:48, 3 August 2011
Wednesday
Lab work
- Ligation of A2,2 and B1 in PSB1C3 O/N 4 degrees.
- Run gel on the PCR products from yesterday: B1 (old) in expr. vector, B1 (new) in PSB1C3 again, A1 in PSB1C3. It seems that either the primers for the B1's arent working - or B1 is not insertet in the plasmids. There was a positive result for A1 - maybe we've got our first biobrick made!! Yayyyy:D
Other work
Checked out how the User Cloning system works. We will use this method on our human Cytochrome P450s. The method is now being optimized by the DTU-Denmark 2 team, and we are therefore getting help from them.
[http://www.neb.com/nebecomm/products/productE5500.asp NEB User Cloning Kit]
Back to Notebook
