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Team:Caltech/Week 8
From 2011.igem.org
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Gibson assemble pNT003 using the gel extracted insert and linearized pSB4A5<br/> | Gibson assemble pNT003 using the gel extracted insert and linearized pSB4A5<br/> | ||
Replating of more pUC19-transformed competent cells and non-transformed competent cells<br/> | Replating of more pUC19-transformed competent cells and non-transformed competent cells<br/> | ||
| + | ===Results=== | ||
| + | <gallery> | ||
| + | File:8-2pcrgel1.jpg|lane 1 NEB 2-log ladder, 2-4 pNT003 insert, 5-7 pNT003 insert with PIPE primers, 8-13 pSB3K3, 14-15 pETDEST53 | ||
| + | File:8-2pcrgel2.jpg|lane 1 NEB 2-log ladder, 2-4 pNT003 insert, 5-7 pNT003 inset with PIPE primers, 8-9 pNT003 insert, 10-11 pNT003 inset with PIPE primers, 12-15 pETDEST53 | ||
| + | </gallery> | ||
}} | }} | ||
Revision as of 23:37, 2 August 2011
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Project |
July 31Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team ResultsMass spectrometry did not run correctly; samples did not ionize; must rerun August 1PCR pSB3K3 and pNT003 insert with the new primers. ResultsNo colonies visible on the pUC19-transformed cells The enrichment culture plates show no visible growth yet. Gel Extractions of July 29 PCR of pNT002 and pNT003 inserts
August 2Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3 Results
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