Team:Caltech/Week 8
From 2011.igem.org
Line 4: | Line 4: | ||
==July 31== | ==July 31== | ||
Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team<br/> | Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team<br/> | ||
+ | ===Results=== | ||
+ | Mass spectrometry did not run correctly; samples did not ionize; must rerun | ||
==August 1== | ==August 1== | ||
Line 13: | Line 15: | ||
===Results=== | ===Results=== | ||
+ | No colonies visible on the pUC19-transformed cells<br/> | ||
<p>The enrichment culture plates show no visible growth yet.</p> | <p>The enrichment culture plates show no visible growth yet.</p> | ||
Gel Extractions of July 29 PCR of pNT002 and pNT003 inserts | Gel Extractions of July 29 PCR of pNT002 and pNT003 inserts | ||
Line 33: | Line 36: | ||
Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3<br/> | Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3<br/> | ||
Gibson assemble pNT003 using the gel extracted insert and linearized pSB4A5<br/> | Gibson assemble pNT003 using the gel extracted insert and linearized pSB4A5<br/> | ||
+ | Replating of more pUC19-transformed competent cells and non-transformed competent cells<br/> | ||
}} | }} |
Revision as of 22:30, 2 August 2011
Project |
July 31Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team ResultsMass spectrometry did not run correctly; samples did not ionize; must rerun August 1PCR pSB3K3 and pNT003 insert with the new primers. ResultsNo colonies visible on the pUC19-transformed cells The enrichment culture plates show no visible growth yet. Gel Extractions of July 29 PCR of pNT002 and pNT003 inserts
August 2Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3
|