Team:Freiburg/Notebook/5 July

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Purification

Investigators: Julia

Purification of digested PCR products with the PCR purification-Kit.

green light receptor

Ligation of Ccas in pSB1C3

Investigators: Julia

Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.

Transformation of PCR product Ccas

Investigators: Jakob

Comments: Colonies had to be picked.

blue light receptor

Transformation of Lov-Tap

Investigators: Jakob

• Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
• Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
• Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)

Precipitator

Gel of digested parts and ligation

Investigators: Rügiger

Gel of digested parts:

• Vector: pSB1K3
• GFP-plastic-binding-domain
• Promotor-RBS (PR1-PR6)

Comments: GFP was excised. Other inserts were not stained properly.

Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.