"
Team:Paris Liliane Bettencourt/Notebook/2011/08/02/
From 2011.igem.org
(Difference between revisions)
(Created page with "==Adrien Lhomme-Duchadeuil== First, an estimation of how much antibiotics is necessary for Bacillus subtilis. Appropriate concentration in antibiotics Chloramphemicol : 5-10 µg/...") |
|||
| Line 2: | Line 2: | ||
First, an estimation of how much antibiotics is necessary for Bacillus subtilis. | First, an estimation of how much antibiotics is necessary for Bacillus subtilis. | ||
Appropriate concentration in antibiotics | Appropriate concentration in antibiotics | ||
| - | Chloramphemicol : 5-10 µg/mL | + | *Chloramphemicol : 5-10 µg/mL |
| - | Spec : 50-100 µg/mL | + | *Spec : 50-100 µg/mL |
| - | Tetracyclin: 25 µg/mL | + | *Tetracyclin: 25 µg/mL |
| - | Kanamycin: 5µg/mL | + | *Kanamycin: 5µg/mL |
| - | Evm(?): 1µg/mL + 25 µg/mL lincomycin (Verify spelling) | + | *Evm(?): 1µg/mL + 25 µg/mL lincomycin (Verify spelling) |
| - | Results of electroporation: IT WORKED after three trials | + | Results of electroporation: IT WORKED after three trials! |
| + | *Positive control worked | ||
| + | *Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance. | ||
| + | * Cm resistant strain have survived in low quatities at both 30 and 5 µg/mL of antibiotics | ||
| + | *It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't all the bacteria. | ||
| + | |||
| + | *To come: protocol and table of results | ||
Revision as of 10:01, 2 August 2011
Adrien Lhomme-Duchadeuil
First, an estimation of how much antibiotics is necessary for Bacillus subtilis. Appropriate concentration in antibiotics
- Chloramphemicol : 5-10 µg/mL
- Spec : 50-100 µg/mL
- Tetracyclin: 25 µg/mL
- Kanamycin: 5µg/mL
- Evm(?): 1µg/mL + 25 µg/mL lincomycin (Verify spelling)
Results of electroporation: IT WORKED after three trials!
- Positive control worked
- Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance.
- Cm resistant strain have survived in low quatities at both 30 and 5 µg/mL of antibiotics
- It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't all the bacteria.
- To come: protocol and table of results
