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From 2011.igem.org

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Revision as of 20:43, 19 March 2011

Materials and Equipment for iGEM.

Here is a list that helps you prepare for the iGEM competition. This list has the materials needed and the equipment that will be used in the iGEM competition. Some of the reagents are generic because the project will specify what type of reagent you will use. Also the list is divided by the type of organism you could use for the competition.

Yeast

• Materials: this list is a beginning point for the lab. There are protocols for Ecoli and Yeast on openwetware that are helpful.
  • Growth media: YPD
  • Selective media: SD with amino acids added
  • Agar: YPD, Electrophoresis agar
  • Restriction enzymes: Biobrick kit from New England Science will help
  • Buffers: TAE/TBE/or LB
  • Seed DNA
  • Stains for gels
  • Enzymes: DNA and RNA Polymerase (if needed), ligase, protease
  • dNTP’s
  • Chemicals: MgCl, Tris-HCl

Equipment for the lab PCR, pressure cooker is being used as an autoclave, Micropipettes and tips, hemocytometer or spectrophotometer, Tubes, plates, Incubator, Shaking water bath or shaking incubator.

Ecoli

• Materials: this list is a beginning point for the lab. There are protocols for Ecoli and Yeast on openwetware that are helpful.
  • Growth Media: T broth, M9, LB
  • Selective Media: Antibiotics added to media
  • Agar: LB agar, Electrophoresis agar
  • Restriction enzymes: Biobrick kit from New England Science will help
  • Buffers: TAE/TBE/or LB
  • Stains for gels
  • Enzymes: DNA and RNA Polymerase (if needed), ligase, protease
  • dNTP’s
  • Chemicals: MgCl, Tris-HCl

Equipment for the lab PCR, pressure cooker is being used as an autoclave, Micropipettes and tips, hemocytometer or spectrophotometer, Tubes, plates, Incubator, Shaking water bath or shaking incubator.