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From 2011.igem.org

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Revision as of 01:25, 1 August 2011

PCR recipe Date: 7/7 Control A Control B Rx1 A Rx1 B Rx2 A Rx2 B Rx3 A Rx3 B Rx4 A Rx4 B Rx5 A Rx5 B Polymerase (pfu Ultra) 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 10X buffer 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul Forward primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul Reverse primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul Plasmid 2ul 2ul 0.5ul 0.5ul 1ul 1ul 5ul 5ul 10ul 10ul 15ul 15ul dNTPs 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul Nuclease free water 34.5ul 34.5ul 26ul 26ul 25.5ul 25.5ul 21.5ul 21.5ul 16.5ul 16.5ul 11.5ul 11.5ul

this it the recipe for the pcr reaction of mutagenesis we did on july 7 thursday.
Cylcle                    temperature         time

Initial warmup 95 degrees 2 mins Denaturation 95 degrees 30 sec annealing 61.8 degrees 30 sec extension 72 degrees 5min final extension 72 degrees 10 min store 4 degrees infinitive

Bivor