"
Team:Imperial College London/Project/Auxin/Notebook
From 2011.igem.org
(Difference between revisions)
(Created page with "<html> <h2>29th of July</h2> Our first transformations were a success! We have managed to transform our competent cell colonies with pSB1C3 containing BBa_K398500 and J23100 prom...") |
|||
| Line 2: | Line 2: | ||
<h2>29th of July</h2> | <h2>29th of July</h2> | ||
Our first transformations were a success! We have managed to transform our competent cell colonies with pSB1C3 containing BBa_K398500 and J23100 promoter to produce cell line 6. We also transformed the cells with part BBa_K316001 to produce cell line 7 and part BBa_K316005 to produce cell line 8. With these cell lines we will be able to make more copies of each part in preparation for the arrival of our synthesized sequences. We are under a tight schedule so efficiency is key.<br><br> | Our first transformations were a success! We have managed to transform our competent cell colonies with pSB1C3 containing BBa_K398500 and J23100 promoter to produce cell line 6. We also transformed the cells with part BBa_K316001 to produce cell line 7 and part BBa_K316005 to produce cell line 8. With these cell lines we will be able to make more copies of each part in preparation for the arrival of our synthesized sequences. We are under a tight schedule so efficiency is key.<br><br> | ||
| - | [Insert photo of three cell lines here] | + | [Insert photo of three cell lines here]<br><br> |
| - | Also, the cell line with the superfolded GFP integrated in its genome has been plated successfully. We have confirmed that these are the correct cell lines by looking at them under UV light. Their green glow was brighter than we expected. We | + | Also, the cell line with the superfolded GFP integrated in its genome has been plated successfully. We have confirmed that these are the correct cell lines by looking at them under UV light. Their green glow was brighter than we expected.<br> |
| + | However, cell line 1 did not grow in the liquid broth media at the same concentration of kanamycin. We will create an assay to ascertain the optimum kanamycin concentration. This is both a bizarre and unexpected result that must be rectified. | ||
</html> | </html> | ||
Revision as of 09:45, 29 July 2011
29th of July
Our first transformations were a success! We have managed to transform our competent cell colonies with pSB1C3 containing BBa_K398500 and J23100 promoter to produce cell line 6. We also transformed the cells with part BBa_K316001 to produce cell line 7 and part BBa_K316005 to produce cell line 8. With these cell lines we will be able to make more copies of each part in preparation for the arrival of our synthesized sequences. We are under a tight schedule so efficiency is key.[Insert photo of three cell lines here]
Also, the cell line with the superfolded GFP integrated in its genome has been plated successfully. We have confirmed that these are the correct cell lines by looking at them under UV light. Their green glow was brighter than we expected.
However, cell line 1 did not grow in the liquid broth media at the same concentration of kanamycin. We will create an assay to ascertain the optimum kanamycin concentration. This is both a bizarre and unexpected result that must be rectified.
