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Sunday, July 24
From 2011.igem.org
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| + | took 1 ul of each of products after npt1 digestion done last thursday, put on ice, added 25 ul of competent cells to each, shocked at 42C for 30 seconnds, placed back on ice for 1 half hour, put onto amp plates, labelled according to products 1B, 2B, 4B 5A and 5B, together with positive and negative controls (seven plates in all) into warm room. On Monday, got plates out of warm room, put into cold room late (4:00 pm)Notes for editing: addition of L when?, lost original notes put on wiki on sunday, 4B was a short draw of competent cells. What kind of competent cells? post transformation protocol, or get link for it. | ||
{{LabReportBmore}} | {{LabReportBmore}} | ||
Revision as of 23:40, 28 July 2011
took 1 ul of each of products after npt1 digestion done last thursday, put on ice, added 25 ul of competent cells to each, shocked at 42C for 30 seconnds, placed back on ice for 1 half hour, put onto amp plates, labelled according to products 1B, 2B, 4B 5A and 5B, together with positive and negative controls (seven plates in all) into warm room. On Monday, got plates out of warm room, put into cold room late (4:00 pm)Notes for editing: addition of L when?, lost original notes put on wiki on sunday, 4B was a short draw of competent cells. What kind of competent cells? post transformation protocol, or get link for it.
Hours
