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Thursday, July 28
From 2011.igem.org
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The contents of these tubes was vortex-ed and poured into its corresponding centerfuge tube above each tube was rinsed with about 1 ml nuclease free water and poured into the corresponding centrifuge tubes. | The contents of these tubes was vortex-ed and poured into its corresponding centerfuge tube above each tube was rinsed with about 1 ml nuclease free water and poured into the corresponding centrifuge tubes. | ||
| - | These six centerfuge tubes were subsequently centerfuged at 4 degrees C. at 12, | + | These six centerfuge tubes were subsequently centerfuged at 4 degrees C. at 12,000 rpm for 30seconds twice. |
each tube was then decanted and wicked without disturbing the supernatant. | each tube was then decanted and wicked without disturbing the supernatant. | ||
6 mLs of Solution I was added to each tube and vortex-ed to re-suspend the pellet. | 6 mLs of Solution I was added to each tube and vortex-ed to re-suspend the pellet. | ||
Revision as of 22:41, 28 July 2011
LABLED 6 CENTERFUGE TUBES
T3 R3 P3
T4 R4 P4
Removed 6 mutagenesis tubes from freezer labled as Mutagenesis P,R and T products grown up and spun down
Lysis by Alkaly
The contents of these tubes was vortex-ed and poured into its corresponding centerfuge tube above each tube was rinsed with about 1 ml nuclease free water and poured into the corresponding centrifuge tubes.
These six centerfuge tubes were subsequently centerfuged at 4 degrees C. at 12,000 rpm for 30seconds twice. each tube was then decanted and wicked without disturbing the supernatant.
6 mLs of Solution I was added to each tube and vortex-ed to re-suspend the pellet.
