Team:Imperial College London/Project/Chemotaxis/Notebook
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Revision as of 21:05, 28 July 2011
28th of July
-We have made tryptone broth, which will be used to grow E. coli (K-12 D10b strain) overnight, for them to developflagella and therefore they will be capable of chemotaxis. To make tryptone broth look at protocols.
-Cells have been transformed with backbone plasmid pSB1C3 carrying biobrick BBa_K398500, with constitutive promoter J23100,
protocol for transformation can be found here.