Team:UNIPV-Pavia/Calendar/July/settimana5

July, 25th

Red colonies were observed from E36 plates, suggesting that the transformation was successful. A colony was picked form the plate.

Digestions of previously purified plasmids were performed for ligations:

Reactions were incubated at 37°C for three hours while a small-size and a medium-size agarose gel were prepared according to protocols.

In the afternoon gel electrophoresis was performed.

Immagine della corsa su gel

After gel extraction, digested DNA was quantified:

Previously purified plasmids carrying either E17, or E18, or E19 or E20 part were inoculated in order to amplify them for the subsequent transformation in MGZ1 cells (E' GIUSTO?):

July, 26th

Plasmids containing either E17, or E18, or E19, or E20 or E36 part were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:

Digestion of E36 part carrying plasmid was performed for ligation:

Digestions of previously purified plasmids were performed for ligations:

Reactions were incubated at 37°C for three hours while a small-size agarose gel was prepared according to protocols.

In the afternoon gel electrophoresis was performed.

Immagine della corsa su gel

After gel extraction, digested DNA was quantified:

Then ligations were performed in a final volume of 10 μl: