Team:Freiburg/Notebook/28 July

From 2011.igem.org

(Difference between revisions)
(→Digestion of PCR product)
(→Digestion of PCR product)
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Incubation for 30 min 37°C.<br/>
Incubation for 30 min 37°C.<br/>
Afterwards the digested product was loaded onto gel.<br/>
Afterwards the digested product was loaded onto gel.<br/>
 +
<br/>
 +
 +
===Miniprep of Not-Gate===
 +
 +
'''Investigators: Sophie'''
 +
 +
Picked colonies from plate and then performed a miniprep.
 +
 +
'''DNA-concentration measured with nanodrop:'''
 +
 +
{| cellpadding="10" cellspacing="0" border="1"
 +
|sample
 +
|DNA-concentration
 +
|-
 +
|M45a
 +
|248.9
 +
|-
 +
|M45b
 +
|256.8
 +
|-
 +
|M45c
 +
|291.3
 +
|-
 +
|M45d
 +
|295.8
 +
|}
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 09:47, 28 July 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Digestion of PCR product

Investigators:Sandra

Digest of PCR product S35 (Lov-tap), because PCR did not work out. We got a band of 1000bp, but we expected 3000bp.
Digest:

  • 1 μl NEB4 buffer
  • 1 μl BSA
  • 1 μl NcoI
  • 2 μl H2O
  • 5 μl S35

Incubation for 30 min 37°C.
Afterwards the digested product was loaded onto gel.

Miniprep of Not-Gate

Investigators: Sophie

Picked colonies from plate and then performed a miniprep.

DNA-concentration measured with nanodrop:

sample DNA-concentration
M45a 248.9
M45b 256.8
M45c 291.3
M45d 295.8

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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