Revision as of 07:52, 28 July 2011

Week 8

From Monday the 1st of August to Friday the 5th of August 2011

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-     <p style="text-align : center"> <small> From Monday the 25th of July to Friday the 29th of July 2011 </small> </p>
+     <p style="text-align : center"> <small> From Monday the 1st of August to Friday the 5th of August 2011 </small> </p>
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-Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.<br/> <br/>
+    <br/> <br/>
-Miniprep of bacteria with mutated part CsgAB. Digestion to check restriction profile (removal of intern PstI site).
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-Send of plasmid with the right profile for part CsgAB to sequencing. Extraction of new MC4100 strain's DNA. PCR with fresh DNA and Taq Phusion.
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-PCR did not work.
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