Team:Cornell/Week 5

From 2011.igem.org

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==Friday==
==Friday==
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Lab work done by: James Mathew & Nancy Li
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Lab work done by: James Mathew and Nancy Li
*GFP PCR- ed off backbone,
*GFP PCR- ed off backbone,
*Primer pair A and B were phosphorylated and annealed
*Primer pair A and B were phosphorylated and annealed

Revision as of 17:14, 27 July 2011

Results | Protocol | Notebook | Parts Submitted

Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |


July 3rd - July 9th

Sunday

Monday

Tuesday

  • Met with our adviser Matt and agreed on final design. Implemented the design into finalized cad tool.

Wednesday

  • Submitted final microfluidic design.

Thursday

  • Put together CSS template for wiki homepage and content pages, as well as starting to make graphics

Friday

Lab work done by: James Mathew and Nancy Li

  • GFP PCR- ed off backbone,
  • Primer pair A and B were phosphorylated and annealed

Saturday

Lab work done by: James Mathew & Charlie Chung

  • GFP PCR product Gel Purified, Digested, PCR clean up
  • pZE-12 Backbone plasmid was miniprepped, digested, Gel Purified
  • Primer pair A and B were phosphorylated and annealed
  • Ligation of all three components done overnight.