Team:Lyon-INSA-ENS/Realisation/Week3

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Revision as of 14:24, 27 July 2011










Week 3


From Monday the 20th of June to Friday the 24th of June 2011





Monday

One transformed colony seemed correct, the others were probably satellite colonies without the plasmid.
Start of 5mL liquid culture of the main colony.
Plating of the satellite colonies to check the presence of the plasmid



Tuesday

4 more new clones have grown : start of 5mL liquid cultures on these clones.



Wednesday

Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)



Thursday

PCR from genomic DNA from E.coli strain MC4100 for three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)



Friday


Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Week3"