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Team:Lyon-INSA-ENS/Realisation/Week4
From 2011.igem.org
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| - | <h6 style="text-align :left"> Tuesday </h6> | + | <h6 style="text-align :left"> Tuesday </h6> <HR> |
<p style = "line-height : 1.5em"> | <p style = "line-height : 1.5em"> | ||
Culture of NM522 cells for later transformations and Curli for extraction. | Culture of NM522 cells for later transformations and Curli for extraction. | ||
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| - | <h6 style="text-align :left"> Wednesday </h6> | + | <h6 style="text-align :left"> Wednesday </h6> <HR> |
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Miniprep, digestion and electrophoresis of the Curli plasmid. | Miniprep, digestion and electrophoresis of the Curli plasmid. | ||
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| - | <h6 style="text-align :left"> Thursday </h6> | + | <h6 style="text-align :left"> Thursday </h6> <HR> |
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CaCl2 chemical transformation of some iGEM kit distribution DNA : <br/><br/> | CaCl2 chemical transformation of some iGEM kit distribution DNA : <br/><br/> | ||
| - | Plate 1 :<br/> | + | <U>Plate 1 : </U><br/> |
18A (constitutive promoter, Amp )<br/> | 18A (constitutive promoter, Amp )<br/> | ||
2M ( strong RBS, Amp ) <br/> | 2M ( strong RBS, Amp ) <br/> | ||
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22M ( RBS+YFP, Amp)<br/><br/> | 22M ( RBS+YFP, Amp)<br/><br/> | ||
| - | Plate 2 :<br/> | + | <U>Plate 2 : </U><br/> |
24E (YFP, Amp + Kan ) <br/> | 24E (YFP, Amp + Kan ) <br/> | ||
2L (GFP, Amp ) <br/><br/> | 2L (GFP, Amp ) <br/><br/> | ||
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<p style = "line-height : 1.5em"> | <p style = "line-height : 1.5em"> | ||
The local newspaper "VIVA" interviewed us. They ask about our project, our team and about iGEM's organization. | The local newspaper "VIVA" interviewed us. They ask about our project, our team and about iGEM's organization. | ||
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| - | <h6 style="text-align :left"> Friday </h6> | + | <h6 style="text-align :left"> Friday </h6> <HR> |
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Revision as of 14:48, 26 July 2011
Week 4
From Monday the 27th of June to Friday the 1st of July 2011
Monday
Tuesday
Culture of NM522 cells for later transformations and Curli for extraction.
Wednesday
Miniprep, digestion and electrophoresis of the Curli plasmid.
Thursday
CaCl2 chemical transformation of some iGEM kit distribution DNA :
Plate 1 :
18A (constitutive promoter, Amp )
2M ( strong RBS, Amp )
5L ( weak RBS, Amp )
22M ( RBS+YFP, Amp)
Plate 2 :
24E (YFP, Amp + Kan )
2L (GFP, Amp )
The local newspaper "VIVA" interviewed us. They ask about our project, our team and about iGEM's organization.
Friday
Miniprep of 6 iGEM parts. Digestion and electrophoresis.
