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Team:Lyon-INSA-ENS/Realisation/Week4
From 2011.igem.org
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| - | + | CaCl2 chemical transformation of some iGEM kit distribution DNA : <br/><br/> | |
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| + | Plate 1 :<br/> | ||
| + | 18A (constitutive promoter, Amp )<br/> | ||
| + | 2M ( strong RBS, Amp ) <br/> | ||
| + | 5L ( weak RBS, Amp )<br/> | ||
| + | 22M ( RBS+YFP, Amp)<br/><br/> | ||
| + | |||
| + | Plate 2 :<br/> | ||
| + | 24E (YFP, Amp + Kan ) <br/> | ||
| + | 2L (GFP, Amp ) <br/><br/> | ||
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Revision as of 13:20, 26 July 2011
Week 4
From Monday the 27th of June to Friday the 1st of July 2011
Monday
Tuesday
Culture of NM522 cells for later transformations and Curli for extraction.
Wednesday
Miniprep, digestion and electrophoresis of the Curli plasmid.
Thursday
CaCl2 chemical transformation of some iGEM kit distribution DNA :
Plate 1 :
18A (constitutive promoter, Amp )
2M ( strong RBS, Amp )
5L ( weak RBS, Amp )
22M ( RBS+YFP, Amp)
Plate 2 :
24E (YFP, Amp + Kan )
2L (GFP, Amp )
The local newspaper "VIVA" interviewed us. They ask about our project, our team and about iGEM's organization.
Friday
Miniprep of 6 iGEM parts. Digestion and electrophoresis.
