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Copenhagen/26 July 2011
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(Created page with "'''Morning''' * The Transformed A2-1 and A2-2 showed no colonies * The Gel done for yesterdays PCR showed no bands '''Lab Work''' *A new ligation of A2-1 and A2-2 with PSB1C3 w...")
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Revision as of 12:15, 26 July 2011
Morning
- The Transformed A2-1 and A2-2 showed no colonies
- The Gel done for yesterdays PCR showed no bands
Lab Work
- A new ligation of A2-1 and A2-2 with PSB1C3 was performed and they were transformed into XL1-BLUE cells
- Digested more PSB1C3
- PCR run on the B1 colonies in the expression vector
- Plasmid prep was done on the overnight cultures
- Digestion of Plasmid prep with EcoRI and PstI
