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Team:Freiburg/Notebook/22 July
From 2011.igem.org
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==Meeting== | ==Meeting== | ||
| - | attendants: | + | attendants: Jakob, Julia, Manuel, Ruediger, Sandra, Sophie, Theo (later), Tobi |
| + | time: 9:00 - 10:30 | ||
===<span style="color:green;">green light receptor</span>=== | ===<span style="color:green;">green light receptor</span>=== | ||
already done: | already done: | ||
| - | + | *CcaS was amplified again from the genomic DNA | |
| - | + | ||
To-do: | To-do: | ||
| - | + | *3-A-Assembly of CcaR into an empty vector with a medium promotor | |
| + | *3-A-Assembly of CcaS into an empty vector with a weak promotor | ||
| + | *3-A-Assembly of CcaR and CcaS to get the final construct | ||
===<span style="color:blue;">blue light receptor</span>=== | ===<span style="color:blue;">blue light receptor</span>=== | ||
| Line 16: | Line 18: | ||
already done: | already done: | ||
| - | + | *Primer-Design for the Gibson-Assembly | |
| - | + | *growth medium without tryptophane is already ordered | |
To-do: | To-do: | ||
| + | *amplify the NOT-Gate | ||
===<span style="color:red;">red light receptor</span>=== | ===<span style="color:red;">red light receptor</span>=== | ||
already done: | already done: | ||
| + | *one positive colony from the 3-A of ho1 and the terminator | ||
| + | *no positive colony from the 3-A of pcyA and the terminator | ||
| + | *the PCR to amplify cph8 didn't give a product, we asked the team from Mexico to send us the cph8 | ||
| + | *the team from Upsala (Sweden) has succeeded in amplifying the cph8 (according to their notebook) | ||
| + | To-do: | ||
| + | *3-A-Assembly of the ho1-term with a mediumPromotor-mediumRBS | ||
| - | |||
| - | |||
===<span style="color:orange;">Lysis cassette</span>=== | ===<span style="color:orange;">Lysis cassette</span>=== | ||
already done: | already done: | ||
| - | + | *the quick change didn't work the first time, we should repeat this | |
| + | *possible alternative to express the lysis cassette could be: temperature regulated RNA | ||
To-do: | To-do: | ||
| - | + | *repeat the quick change | |
===<span style="color:grey;">Precipitator</span>=== | ===<span style="color:grey;">Precipitator</span>=== | ||
already done: | already done: | ||
| + | *the GFP-PBD was cloned (colonies showed up) | ||
To-do: | To-do: | ||
| + | *test digest and sequencing of the GFP-PBD (first have a look if you see the GFP fluorescence) | ||
| + | *possible test of the PBD: grow the E. coli, lyse them, put the lysate into a plate (96 well?), wash the wells to get rid of the other proteins, measure the GFP fluorescence with a plate reader | ||
| + | *microscope the E. coli cells | ||
| + | |||
| + | ===other suff</span>=== | ||
| + | *think about possible give-aways for the sponsoring video | ||
| + | *jacob will create a Youtube account and upload the sponsoring video | ||
| + | *meeting for the wiki takeover: wednesday 9:00 am (new time: 12:15) | ||
| + | *modelling should be started now, either Ni-binding and release or GFP-PBD binding and release, Ruediger will do this | ||
| + | |||
<br/> | <br/> | ||
<br/> | <br/> | ||
Revision as of 08:16, 26 July 2011
Contents |
Meeting
attendants: Jakob, Julia, Manuel, Ruediger, Sandra, Sophie, Theo (later), Tobi time: 9:00 - 10:30
green light receptor
already done:
- CcaS was amplified again from the genomic DNA
To-do:
- 3-A-Assembly of CcaR into an empty vector with a medium promotor
- 3-A-Assembly of CcaS into an empty vector with a weak promotor
- 3-A-Assembly of CcaR and CcaS to get the final construct
blue light receptor
already done:
- Primer-Design for the Gibson-Assembly
- growth medium without tryptophane is already ordered
To-do:
- amplify the NOT-Gate
red light receptor
already done:
- one positive colony from the 3-A of ho1 and the terminator
- no positive colony from the 3-A of pcyA and the terminator
- the PCR to amplify cph8 didn't give a product, we asked the team from Mexico to send us the cph8
- the team from Upsala (Sweden) has succeeded in amplifying the cph8 (according to their notebook)
To-do:
- 3-A-Assembly of the ho1-term with a mediumPromotor-mediumRBS
Lysis cassette
already done:
- the quick change didn't work the first time, we should repeat this
- possible alternative to express the lysis cassette could be: temperature regulated RNA
To-do:
- repeat the quick change
Precipitator
already done:
- the GFP-PBD was cloned (colonies showed up)
To-do:
- test digest and sequencing of the GFP-PBD (first have a look if you see the GFP fluorescence)
- possible test of the PBD: grow the E. coli, lyse them, put the lysate into a plate (96 well?), wash the wells to get rid of the other proteins, measure the GFP fluorescence with a plate reader
- microscope the E. coli cells
other suff</span>
- think about possible give-aways for the sponsoring video
- jacob will create a Youtube account and upload the sponsoring video
- meeting for the wiki takeover: wednesday 9:00 am (new time: 12:15)
- modelling should be started now, either Ni-binding and release or GFP-PBD binding and release, Ruediger will do this
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
