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Team:Paris Liliane Bettencourt/Notebook/2011/07/22/
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(Created page with "== Cyrille == Prearation of the pHM3 plasmid. This plasmid from H. Pfutzer contains 2 EcoRI excision site. One will be removed by quick change mutagenesis. 42 base oligo couple...")
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(Created page with "== Cyrille == Prearation of the pHM3 plasmid. This plasmid from H. Pfutzer contains 2 EcoRI excision site. One will be removed by quick change mutagenesis. 42 base oligo couple...")
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Revision as of 14:33, 25 July 2011
Cyrille
Prearation of the pHM3 plasmid. This plasmid from H. Pfutzer contains 2 EcoRI excision site. One will be removed by quick change mutagenesis.
42 base oligo couple has been ordered from eurogentec. The protocol is the one http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/quick_change.htmlhere
