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Team:Freiburg/Notebook/25 July
From 2011.igem.org
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'''PCR''' | '''PCR''' | ||
Revision as of 10:25, 25 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
- Gibson NOT-Gate
Investigators:
- Sophie
PCR
| Name: Sophie
| Date: 25.07.11 |
| Continue from Experiment: new experiment. We need NOT-Gate and LovTAP with Gibson-overhangs, so 2 PCRs are made | |
| Project Name: Blue Light | |
PCR-Mixture for one Reaction:
For a 50 µl reaction use
| 32,5µl | H20 | Name |
| 10µl | 5x Phusion Buffer | of Primer: |
| 2.5µl | Primer fw | NOT_G_up
LOV_G_up |
| 2.5µl | Primer dw | NOT_G_dw
LOV_G_dw |
| 1µl | dNTPs | of Template DNA |
| 1µl | DNA-Template | S35 (BBa_K322999)
S45 (BBa_Q04400) |
| 0.5 µl | Phusion (add in the end) |
What program do you use?
67c auf 70c
To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.
How did you label the PCR-Product, where is it stored and what do you do next?
I labelled it with a heart and a star and stored it in the minipreps 3 box.
I will do Gibson assemblz of the two parts next.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
