Team:Grinnell/Notebook/Gels/Promoters

From 2011.igem.org

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==2011.06.19-2011.06.25==
==2011.06.19-2011.06.25==
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<a name='20110620_promotorsTransformationGel' href='https://2011.igem.org/File:20110620_promotorsTransformationGel.jpg'>
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<img alt='20110620_promotorsTransformationGel' src='https://static.igem.org/mediawiki/2011/4/4c/20110620_promotorsTransformationGel.jpg' />
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<a name='20110621_BBaGel1' href='https://2011.igem.org/File:20110621_BBaGel1.jpg'>
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<img alt='20110621 BBaGel1' src='https://static.igem.org/mediawiki/2011/4/43/20110621_BBaGel1.jpg' />
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<a name='20110622_PromotersGel2' href='https://2011.igem.org/File:20110622_PromotersGel2.jpg'>
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<img alt='20110622_PromotersGel2' src='https://static.igem.org/mediawiki/2011/a/a3/20110622_PromotersGel2.jpg' />
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Gel of Plasmid PCR of promoters. Presence of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors.
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Gel of BBa_K081005 digests from minipreps. Lane 1: ladder; lane 2: digest of miniprep from overnights that should carry the desired promoter insert. Digest shows one band: the linearized plasmid.
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Lane 1: ladder; lanes 2-4: plasmid PCR product of various promoter insertions into pSB1C3. Smearing due to leftover circular plasmid. Insertions seem to have been successful.
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==2011.06.26-2011.07.02==
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Revision as of 16:15, 22 July 2011

Grinnell Menubar

Gels for Promoters

2011.06.12-2011.06.18

20110614_Promoters 20110616_promotorGel 20110616_promoterPostCleanupCrop
Gel of PCR of Caulobacter promoters Pxyl (inducible) and PrsaA (constitutive). Lane 1: ladder; lane 2: PrsaA; Lane 3: Pxyl. Bands appear, but are hazy and spread out due to the small size of DNA fragments. PCR products of Caulobacter promoters PrsaA and Pxyl. Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl. Gel confirming that clean up did not remove DNA fragments. Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl.

2011.06.19-2011.06.25

20110620_promotorsTransformationGel 20110621 BBaGel1 20110622_PromotersGel2
Gel of Plasmid PCR of promoters. Presence of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors. Gel of BBa_K081005 digests from minipreps. Lane 1: ladder; lane 2: digest of miniprep from overnights that should carry the desired promoter insert. Digest shows one band: the linearized plasmid. Lane 1: ladder; lanes 2-4: plasmid PCR product of various promoter insertions into pSB1C3. Smearing due to leftover circular plasmid. Insertions seem to have been successful.

2011.06.26-2011.07.02

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