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Sunday, July 17
From 2011.igem.org
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| + | =Transformation of Dpn1-digested plasmid= | ||
| + | 1pm | ||
| - | + | Transformed contents of tubes 3a, 3b, 4a using [[Team:Baltimore/Protocols/transformation|transformation]] protocol with the following changes: | |
| - | + | * 2µl of mutagenesis mix used instead of 1µl. | |
| + | * LB media instead of SOC. | ||
| - | + | ==Timeline== | |
| + | * Transformanda on ice at 1305h. | ||
| + | * Heat shocked @ 1335h. | ||
| + | * incubating at 37˚C until 1415. | ||
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Retrieved from "https://2011.igem.org/Team:Baltimore/Notebook/Transformation" | Retrieved from "https://2011.igem.org/Team:Baltimore/Notebook/Transformation" | ||
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| + | {{LabReportBmore}} | ||
Latest revision as of 21:23, 21 July 2011
Transformation of Dpn1-digested plasmid
1pm
Transformed contents of tubes 3a, 3b, 4a using transformation protocol with the following changes:
- 2µl of mutagenesis mix used instead of 1µl.
- LB media instead of SOC.
Timeline
- Transformanda on ice at 1305h.
- Heat shocked @ 1335h.
- incubating at 37˚C until 1415.
Retrieved from "https://2011.igem.org/Team:Baltimore/Notebook/Transformation"
Hours
