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JULY: WEEK 4
July, 18th
Digestions of previously purified plasmids were performed for ligations: CAMBIARE OGGETTI TABELLA
Reactions were incubated at 37°C for three hours while two medium-size agarose gels were prepared according to protocols. In the afternoon gel electrophoresis was performed.
2 righe sopra inserire l' immagine As shown, in figure all clones were positive (apart from <partinfo>BBa_I13501</partinfo> run where we inexplicably didn't see any band), so we cut and purified the bands of interest. Cells harbouring <partinfo>BBa_I13501</partinfo> were again inoculated in 5 ml LB + Amp. After gel extraction, cut DNA was quantified:
Then ligations were performed in a final volume of 10 μl:
Ligations were incubated ON at 16°C. July, 19th
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Team:UNIPV-Pavia/Calendar/July/settimana4
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Revision as of 09:16, 21 July 2011
