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Team:Caltech/Week 6
From 2011.igem.org
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==July 18== | ==July 18== | ||
| - | Colony PCR of positive Gibson plates from Friday<br/> | + | Colony PCR of colonies from positive Gibson plates from Friday, for vector and insert<br/> |
| + | Colony PCR of 16s plasmids, for vector and insert<br/> | ||
Gel of short PCR parts to confirm they are being amplified<br/> | Gel of short PCR parts to confirm they are being amplified<br/> | ||
Pulsed field gel with a larger amount of soil sample DNA<br/> | Pulsed field gel with a larger amount of soil sample DNA<br/> | ||
Retransform pSB3K5 into Xl-10s<br/> | Retransform pSB3K5 into Xl-10s<br/> | ||
===Results=== | ===Results=== | ||
| + | [[File:Gel1_7_9_Caltech.jpg|thumb|Colonies 1-6 from Gibson plates; 7 and 8 are reswabs of two colonies. From left to right: DNA ladder,(PNT001; colony 1; vector), P2v, P3v, P4v, P5v, P6v, P7v, P8v, (PNT001; colony 1; insert), P3i, P4i, P5i, P6i]] | ||
| + | [[File:Gel2_7_19_Caltech.jpg.jpg|thumb|from left to right: DNA ladder, (PNT001; reswab 7; insert), P8i, (16s; colony A; vector), 16Bv, 16Cv, (16s; A; insert), 16Bi, 16Ci]] | ||
==July 19== | ==July 19== | ||
PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control<br> | PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control<br> | ||
Revision as of 21:11, 20 July 2011
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Project |
July 18Colony PCR of colonies from positive Gibson plates from Friday, for vector and insert Results  July 19PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control ResultspSB3K5 plate had 0 colonies. Gibson PCR concentrations
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