Uppsala-Sweden/11 July 2011

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2011-07-11
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Today we did a frozen stock of all viable stocks in -80°C freezer (ccaS clone 1,RBS-pcyA, amilGFP, amilCP, ccaR, RBS-ho1, PompC-Inv.cI). We also did a 3ml culture of the two clones of ccaR, RBS-pcyA and RBS-ho1, the constructs needed for the upcoming assemblies. Furthermore, we did a plasmid preparation of this clones. Transformation of tetR and lambda CI from MIT distribution. PCR of amilCP to add BioBrick prefix and suffix. Mutagensis of amilGFP-PstI and ccaS mutagenesis, round one.

Latest revision as of 20:26, 20 July 2011







Notebook

June
MTWTFSS
    [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_June_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_June_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_June_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_June_2011&action=edit 5]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_June_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_June_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_June_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_June_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_June_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_June_2011&action=edit 12]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_June_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_June_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_June_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_June_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_June_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_June_2011&action=edit 19]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_June_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_June_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_June_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_June_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_June_2011&action=edit 26]
[http://2011.igem.org/Uppsala-Sweden/27_June_2011 27] [http://2011.igem.org/Uppsala-Sweden/28_June_2011 28] [http://2011.igem.org/Uppsala-Sweden/29_June_2011 29] [http://2011.igem.org/Uppsala-Sweden/30_June_2011 30]
July
MTWTFSS
        [http://2011.igem.org/Uppsala-Sweden/1_July_2011 1] [http://2011.igem.org/Uppsala-Sweden/2_July_2011 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_July_2011&action=edit 3]
[http://2011.igem.org/Uppsala-Sweden/4_July_2011 4] [http://2011.igem.org/Uppsala-Sweden/5_July_2011 5] [http://2011.igem.org/Uppsala-Sweden/6_July_2011 6] [http://2011.igem.org/Uppsala-Sweden/7_July_2011 7] [http://2011.igem.org/Uppsala-Sweden/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_July_2011&action=edit 10]
[http://2011.igem.org/Uppsala-Sweden/11_July_2011 11] [http://2011.igem.org/Uppsala-Sweden/12_July_2011 12] [http://2011.igem.org/Uppsala-Sweden/13_July_2011 13] [http://2011.igem.org/Uppsala-Sweden/14_July_2011 14] [http://2011.igem.org/Uppsala-Sweden/15_July_2011 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_July_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_July_2011&action=edit 17]
[http://2011.igem.org/Uppsala-Sweden/18_July_2011 18] [http://2011.igem.org/Uppsala-Sweden/19_July_2011 19] [http://2011.igem.org/Uppsala-Sweden/20_July_2011 20] [http://2011.igem.org/Uppsala-Sweden/21_July_2011 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_July_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_July_2011&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_July_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_July_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_July_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_July_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_August_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_August_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_August_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_August_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_August_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_August_2011&action=edit 21]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_August_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_August_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_August_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_August_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_August_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_August_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_August_2011&action=edit 28]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_August_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_August_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_August_2011&action=edit 31]
September
MTWTFSS
      [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_September_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_September_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_September_2011&action=edit 4]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_September_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_September_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_September_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_September_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_September_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_September_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_September_2011&action=edit 11]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_September_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_September_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_September_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_September_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_September_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_September_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_September_2011&action=edit 18]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_September_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_September_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_September_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_September_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_September_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_September_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_September_2011&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_September_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_September_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_September_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_September_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_October_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_October_2011&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_October_2011&action=edit 31]

2011-07-11

Today we did a frozen stock of all viable stocks in -80°C freezer (ccaS clone 1,RBS-pcyA, amilGFP, amilCP, ccaR, RBS-ho1, PompC-Inv.cI). We also did a 3ml culture of the two clones of ccaR, RBS-pcyA and RBS-ho1, the constructs needed for the upcoming assemblies. Furthermore, we did a plasmid preparation of this clones. Transformation of tetR and lambda CI from MIT distribution. PCR of amilCP to add BioBrick prefix and suffix. Mutagensis of amilGFP-PstI and ccaS mutagenesis, round one.
Retrieved from "http://2011.igem.org/Uppsala-Sweden/11_July_2011"