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Uppsala-Sweden/27 June 2011
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Revision as of 15:34, 20 July 2011
2011-06-27
This week we started with testing the competence in our cells. For this transformation we used one positive control (plasmid pUC19 from New England Biolabs) as well as a negative control without plasmid. For the procedure we followed the protocol for transforming TOP10 competent cells. The result was good, we measured a competence efficiency of 1.7 * 10^8 transformants /ug DNA.
Started overnight cultures of E coli carrying the plasmids pGEM11- amilGFP (green output) and pGEM14- amilCP (blue output). For this procedure we followed the protocol Overnight culture and glycerol stock. The strains carrying the amilGFP and amilCP plasmids were provided by J.F Miller, UCLA.
