"
Copenhagen/20 July 2011
From 2011.igem.org
(Difference between revisions)
EvaCar (Talk | contribs)
(Created page with "===Wednesday=== A few mistakes from last week, a fully booked PCR machine and a crowded freezer led to a morning of low spirits and around 12 O'clock it seemed there were nothin...")
Newer edit →
(Created page with "===Wednesday=== A few mistakes from last week, a fully booked PCR machine and a crowded freezer led to a morning of low spirits and around 12 O'clock it seemed there were nothin...")
Newer edit →
Revision as of 11:55, 20 July 2011
Wednesday
A few mistakes from last week, a fully booked PCR machine and a crowded freezer led to a morning of low spirits and around 12 O'clock it seemed there were nothing more that could be done. But after a lunch everything looked much brighter and positive thinking and troubleshooting kept us busy from then on - the A1 was ligated into the standard plasmid AND the expression vector. Around 2 O'clock another miracle happened as a missing BioBrick disappeared in the freezer and made sure that we had something to do for the rest of the day as well.
A1 The only one without any problems.
- Double Digestion with Xba1 and Pst1
- Ligation with the expression vector
- Size of BioBrick J04500 = 220bp+3189=3409 bp
- Size of BioBrick A1 = 1600bp
- 3409/1600= ca. 2
- Transformation
Expressionvector'
- Double Digestion with Pst1 and Spe1
