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Team:British Columbia/Notebook/18 July 2011
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(Created page with "====beta-pinene==== *sequencing failed for all of P2SDM1, P2SDM2, P2SDM3. *restriction digested the miniprepped plasmids to see if the plasmids get cut (if the site is still the...")
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(Created page with "====beta-pinene==== *sequencing failed for all of P2SDM1, P2SDM2, P2SDM3. *restriction digested the miniprepped plasmids to see if the plasmids get cut (if the site is still the...")
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Revision as of 19:04, 19 July 2011
beta-pinene
- sequencing failed for all of P2SDM1, P2SDM2, P2SDM3.
- restriction digested the miniprepped plasmids to see if the plasmids get cut (if the site is still there, then the vector should cut and give a single band).
- gel verification of the digests suggest that the site has been removed (show bands that are identical to the uncut template/original vector).
