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Team:Freiburg/Notebook/19 July
From 2011.igem.org
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Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor<br/> | Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor<br/> | ||
<br/> | <br/> | ||
| - | LOVtap-Trp_up gaattcgcggccgcttctagtcacacaggaaagtactatgt<br/> | + | *LOVtap-Trp_up: gaattcgcggccgcttctagtcacacaggaaagtactatgt<br/> |
| - | LOVtap-Trp_dw tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg<br/> | + | *LOVtap-Trp_dw: tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg<br/> |
| - | TetR-TetO_up aaagaggagaaatactagatgtccagattag<br/> | + | *TetR-TetO_up: aaagaggagaaatactagatgtccagattag<br/> |
| - | TetR-TetO_dw* ctgcagcggccgctactag<br/> | + | *TetR-TetO_dw*: ctgcagcggccgctactag<br/> |
*we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.<br/> | *we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.<br/> | ||
Revision as of 14:13, 19 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Theoretical Gibson-Assembly
Investigators: Sandra, Sophie
Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor
- LOVtap-Trp_up: gaattcgcggccgcttctagtcacacaggaaagtactatgt
- LOVtap-Trp_dw: tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg
- TetR-TetO_up: aaagaggagaaatactagatgtccagattag
- TetR-TetO_dw*: ctgcagcggccgctactag
- we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
