"
Team:Cambridge/Protocols/Transformation of B. subtilis
From 2011.igem.org
(Difference between revisions)
(→Practice) |
(→Safety) |
||
| Line 26: | Line 26: | ||
===Safety=== | ===Safety=== | ||
| - | + | All equipment (including gloves) that may have come into contact with the bacteria must be autoclaved for decontamination purposes. | |
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | ||
Revision as of 13:22, 18 July 2011
Loading...
Protocol Name
Theory
How it works
Practice
- Add to a clean Eppendorf tube:
Reagent Quantity competent Bacillus cells 400μl DNA 3μl
- Incubate at 37ºC for 1 hour.
- Plate on an agar plate containing antibiotic, which allows for selection of successful transformants.
Safety
All equipment (including gloves) that may have come into contact with the bacteria must be autoclaved for decontamination purposes.
