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Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification
From 2011.igem.org
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This protocol is for preparation of up to 200µg of high- or low-copy plasmid or cosmid DNA using the HiSpeed Plasmid Midi Kit. | This protocol is for preparation of up to 200µg of high- or low-copy plasmid or cosmid DNA using the HiSpeed Plasmid Midi Kit. | ||
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A final DNA concentration of up to 0.4µg/µL can be expected, if eluting a high-copy plasmid with 500µL of Buffer TE. eluates from low-copy vectors or dilute samples can be concentrated by spinning in a centrifuge under vacuum, or ethanol precipitation. Low-copy plasmids that have been amplified in the presence of chloramphenicol should be treated as high-copy plamids when choosing the appropriate culture volume. | A final DNA concentration of up to 0.4µg/µL can be expected, if eluting a high-copy plasmid with 500µL of Buffer TE. eluates from low-copy vectors or dilute samples can be concentrated by spinning in a centrifuge under vacuum, or ethanol precipitation. Low-copy plasmids that have been amplified in the presence of chloramphenicol should be treated as high-copy plamids when choosing the appropriate culture volume. | ||
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Revision as of 09:24, 18 July 2011
Plasmid or Cosmid DNA Purification Using HiSpeed Plasmid Midi Kits
This protocol is for preparation of up to 200µg of high- or low-copy plasmid or cosmid DNA using the HiSpeed Plasmid Midi Kit.
A final DNA concentration of up to 0.4µg/µL can be expected, if eluting a high-copy plasmid with 500µL of Buffer TE. eluates from low-copy vectors or dilute samples can be concentrated by spinning in a centrifuge under vacuum, or ethanol precipitation. Low-copy plasmids that have been amplified in the presence of chloramphenicol should be treated as high-copy plamids when choosing the appropriate culture volume.
