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Team:Baltimore/Notebook/Transformation
From 2011.igem.org
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| - | + | =Transformation of Dpn1-digested plasmid= | |
17 July 2011 1pm | 17 July 2011 1pm | ||
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* LB media instead of SOC. | * LB media instead of SOC. | ||
| - | Transformanda on ice at 1305h. | + | ==Timeline== |
| - | Heat shocked @ 1335h | + | * Transformanda on ice at 1305h. |
| + | * Heat shocked @ 1335h. | ||
| + | * incubating at 37˚C until 1415. | ||
Revision as of 17:52, 17 July 2011
Transformation of Dpn1-digested plasmid
17 July 2011 1pm
Transformed contents of tubes 3a, 3b, 4a using transformation protocol with the following changes:
- 2µl of mutagenesis mix used instead of 1µl.
- LB media instead of SOC.
Timeline
- Transformanda on ice at 1305h.
- Heat shocked @ 1335h.
- incubating at 37˚C until 1415.
