Team:NCTU Formosa

From 2011.igem.org

(Difference between revisions)

Revision as of 16:53, 15 July 2011

網頁組，點上方edit就可以編輯了^__^

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	File:NCTU Formosa team.png Your team picture
	Team Example

Team NCTU_Formosa: Pathway Commander

As one of the main challenges of in vivo experiments is controlling the flux through a synthetic metabolic pathway, it’s design lies in selecting well-matched genetic components that when coupled, can reliably produce the desired behavior. Although model equations can calculate parameter values, a challenge still remains in selecting the bio-bricks that can reliably implement a desired cellular function with quantitative values. In previous studies, synthetic biologists have created numerous synthetic circuits; each generating different protein expression levels in order to test performance in reliability and consistency, but this process is both tedious and time consuming. To overcome this problem, our team designed a novel circuit design: method_ Pathway Commander. By this design method, we construct a single version of a synthetic metabolic pathway circuit that can use culture temperature shifts to control the expression levels of a series of metabolic proteins at the precise times. We have implemented the Pathway_Commander design in (1) Carotenoid synthesis Pathway, (2) Violacein biosynthesis pathways and (3) Butanol synthesis pathway in E. coli. This circuit design utilizes a temperature controlled system that gives precision control over metabolic protein expression which amounts to optimal synthesis that can maximize synthesis of a given compound or drug.

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

@@ Line 29: / Line 29: @@
 |align="center"|[[Team:NCTU_Formosa | Team Example]]
 |}
-'''Team NCTU_Formosa: Pathway Commander'''/p
+'''Team NCTU_Formosa: Pathway Commander'''
-As one of the main challenges of in vivo experiments is controlling the flux through a synthetic  metabolic pathway, it’s design lies in selecting well-matched genetic components that when coupled, can reliably produce the desired behavior. Although model equations can calculate parameter values, a challenge still remains in selecting the bio-bricks that can reliably implement a desired cellular function with quantitative values. In previous studies, synthetic biologists have created numerous synthetic circuits; each generating different protein expression levels in order to test performance in reliability and consistency, but this process is both tedious and time consuming. To overcome this problem, our team designed a novel circuit design: method_ Pathway Commander. By this design method, we construct a single version of a synthetic metabolic pathway circuit that can use culture temperature shifts to control the expression levels of a series of metabolic proteins at the precise times. We have implemented the Pathway_Commander design in (1) Carotenoid synthesis Pathway, (2) Violacein biosynthesis pathways and (3) Butanol synthesis pathway in E. coli. This circuit design utilizes a temperature controlled system that gives precision control over metabolic protein expression which amounts to optimal synthesis that can maximize synthesis of a given compound or drug.
+As one of the main challenges of'' in vivo'' experiments is controlling the flux through a synthetic  metabolic pathway, it’s design lies in selecting well-matched genetic components that when coupled, can reliably produce the desired behavior. Although model equations can calculate parameter values, a challenge still remains in selecting the bio-bricks that can reliably implement a desired cellular function with quantitative values. In previous studies, synthetic biologists have created numerous synthetic circuits; each generating different protein expression levels in order to test performance in reliability and consistency, but this process is both tedious and time consuming. To overcome this problem, our team designed a novel circuit design: method_ Pathway Commander. By this design method, we construct a single version of a synthetic metabolic pathway circuit that can use culture temperature shifts to control the expression levels of a series of metabolic proteins at the precise times. We have implemented the Pathway_Commander design in (1) Carotenoid synthesis Pathway, (2) Violacein biosynthesis pathways and (3) Butanol synthesis pathway in'' E. coli''. This circuit design utilizes a temperature controlled system that gives precision control over metabolic protein expression which amounts to optimal synthesis that can maximize synthesis of a given compound or drug.