Team:Freiburg/Notebook/15 July

From 2011.igem.org

(Difference between revisions)
(red light receptor)
(red light receptor)
Line 21: Line 21:
To-do:
To-do:
-
==<span style="color:red;">red light receptor</span>==
+
===<span style="color:red;">red light receptor</span>===
already done:
already done:
Line 28: Line 28:
To-do:
To-do:
-
 
-
 
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==

Revision as of 09:15, 15 July 2011

Contents

Meeting

attendants: Tobi, Theo, Julia, Rüdiger, Jakob, Sandra

green light receptor

already done:


To-do:


blue light receptor

already done:


To-do:

red light receptor

already done:


To-do:

Lysis cassette

already done:

  1. repressor part (BBa_K098995) has no bases between RBS (B0034) and start codon (K098997), resulting in no translation.
    • quickchange of the repressor part to insert 6bp between RBS and start codon

To-do:

  1. DpnI digestion to digest the DNA template (methylated DNA) of the PCR to have only the new synthesized DNA strand.
    • After digestion with DpnI, transformation of cells with the corrected part (BBa_K08995)

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME