Team:EPF-Lausanne/Protocols/Plating
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(Created page with "{{:Team:EPF-Lausanne/Templates/Header|title=Plating }} When you incubate transformed cells at 37°C, also put the agar plates to warm them up. After one hour: * Under the flame,...")
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Revision as of 15:03, 14 July 2011
Plating
When you incubate transformed cells at 37°C, also put the agar plates to warm them up. After one hour:
- Under the flame, put 100 ul of cells in the plate
- Put autoclaved beads in the plate and shake it
- Put the beads in the "used" box
- The plates have to stay at 37°C overnight