Experiments were conducted in the labs of Dr. Guhan Jayaraman in the Dept. Of Biotechnology at IIT Madras and Dr. Mukund Thattai at National Centre for Biological Sciences. Both the labs conformed to the standards for a biosafety 1 lab. Biosafety Level 1 is suitable for work involving well-characterized agents not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment. Biosafety Level 1 is suitable for work involving well-characterized agents not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.

Standard Microbiological Practices

1. Access to the laboratory is limited or restricted at the discretion of the laboratory director when experiments or work with cultures and specimens are in progress.
2. Persons wash their hands after they handle viable materials, after removing gloves, and before leaving the laboratory.
3. Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing food for human use are not permitted in the work areas. Persons who wear contact lenses in laboratories should also wear goggles or a face shield. Food is stored outside the work area in cabinets or refrigerators designated and used for this purpose only.
4. Mouth pipetting is prohibited; mechanical pipetting devices are to be used.
5. Policies for the safe handling of sharps are instituted.
6. All procedures are performed carefully to minimize the creation of splashes or aerosols.
7. Work surfaces are decontaminated at least once a day and after any spill of viable material.
8. All cultures, stocks, and other regulated wastes are decontaminated before disposal by an approved decontamination method such as autoclaving. Materials to be decontaminated outside of the immediate laboratory are to be placed in a durable, leakproof container and closed for transport from the laboratory. Materials to be decontaminated outside of the immediate laboratory are packaged in accordance with applicable local, state, and federal regulations before removal from the facility.

Safety Equipment (Primary Barriers)

1. Special containment devices or equipment such as a biological safety cabinet is generally not required for manipulations of agents assigned to Biosafety Level 1.
2. It is recommended that laboratory coats, gowns, or uniforms be worn to prevent contamination or soiling of street clothes.
3. Gloves should be worn if the skin on the hands is broken or if a rash is present. Alternatives to powdered latex gloves should be available.
4. Protective eyewear should be worn for conduct of procedures in which splashes of microorganisms or other hazardous materials is anticipated.

Laboratory Facilities (Secondary Barriers)

1. Laboratory has doors for access control.
2. Laboratory contains a sink for hand washing.
3. The laboratory is designed so that it can be easily cleaned.
4. Bench tops are impervious to water and are resistant to moderate heat and the organic solvents, acids, alkalis, and chemicals used to decontaminate the work surface and equipment.
5. Laboratory furniture is capable of supporting anticipated loading and uses. Spaces between benches, cabinets, and equipment are accessible for cleaning.
6. Laboratory has no windows that open to the exterior.

Safety

1. Would any of your project ideas raise safety issues in terms of:

No

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,

No. All biobricks made have already been tested in multiple organisms and have been found to be safe.

3. Is there a local biosafety group, committee, or review board at your institution?

Yes, our project has the backing of the dept of biotechnology, IIT Madras. They have been provided us with Biosafety level 1 facilities for all our experimental work.

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

As all of the teams are essentially undergraduate efforts, we are to a large extent unfamiliar with the consequences of most safety protocols. We reccomend that as a part of the jamboree a small symposium on the importance of safety practices so that teams who were unable to attend such seminars to learn about these practices.