Team:Freiburg/Notebook/4 July

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(Created page with "=='''23. Labday'''== ---- ==Digestion of PCR products== '''Investigators: Julia''' Digest of PCR product Ccas. Ccas: *Enzyme 1: XbaI *Enzyme 2: PstI in vector pSB1C3: *Enzy...")
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Revision as of 09:12, 14 July 2011

23. Labday


Digestion of PCR products

Investigators: Julia

Digest of PCR product Ccas.

Ccas:

  • Enzyme 1: XbaI
  • Enzyme 2: PstI

in vector pSB1C3:

  • Enzyme 1: SpeI
  • Enzyme 2: PstI

Comments: RFP is not digested with SpeI and PstI. It was digested again, that time with NEB4 and XbaI.