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Team:Cambridge/Blog/Week 3
From 2011.igem.org
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| - | Our mini-projects continue apace with transformation of (hopefully) working plasmids into E. coli for | + | Our mini-projects continue apace with transformation of (hopefully) working plasmids into E. coli for amplification (the bacteria reproduce, making more of our DNA at the same time). The team are introduced to the lab's high-tech portable dark room for working with fluorescence: a customisable, cubic, fully-opaque, stiffened wood-fibre local micro-environment device (known in the trade as a cardboard box). |
The team's ideas converge further and seem to centre on [http://www.ncbi.nlm.nih.gov/pubmed?term=reflectin reflectin] - a protein with highly unusual properties isolated only relatively recently from [http://en.wikipedia.org/wiki/Bobtail_squid a class of squid]. Enthusiasm is higher than ever with a focus for our efforts. | The team's ideas converge further and seem to centre on [http://www.ncbi.nlm.nih.gov/pubmed?term=reflectin reflectin] - a protein with highly unusual properties isolated only relatively recently from [http://en.wikipedia.org/wiki/Bobtail_squid a class of squid]. Enthusiasm is higher than ever with a focus for our efforts. | ||
Revision as of 23:00, 13 July 2011
Week 3 : 11th of July to 17th of July
Monday
The day begins with assembling our amplified DNA into working plasmids in order to transform Bacillus. Several mislabelled tubes and much all-round amateurishness later the task is complete and the day's brainstorming begins. Our possible projects are by now narrowed down to four which show some promise. It seems hopeful that a final choice can be made soon.
Tuesday
Our mini-projects continue apace with transformation of (hopefully) working plasmids into E. coli for amplification (the bacteria reproduce, making more of our DNA at the same time). The team are introduced to the lab's high-tech portable dark room for working with fluorescence: a customisable, cubic, fully-opaque, stiffened wood-fibre local micro-environment device (known in the trade as a cardboard box).
The team's ideas converge further and seem to centre on [http://www.ncbi.nlm.nih.gov/pubmed?term=reflectin reflectin] - a protein with highly unusual properties isolated only relatively recently from [http://en.wikipedia.org/wiki/Bobtail_squid a class of squid]. Enthusiasm is higher than ever with a focus for our efforts.
Wednesday
Thursday
Friday
Saturday
Sunday
