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Team:BU Wellesley Software/Notebook/ShannonNotebook
From 2011.igem.org
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| - | + | == 6/06/2011-6/10/2011 == | |
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| + | * included a boot camp. | ||
| + | * different topics were discussed (i.e. biobricks, gene regulation, differences between eukaryotes and prokaryotes). | ||
| + | * boot camp also included a computer science component (focused on Clotho). | ||
| + | * miniprepes were done on previously transformed BFP2. After minipreps, the Nanodrop was used to quantify the DNA. Minipreps did not have high DNA concentration, but were run on a gel (to show presence of DNA). Most lanes appeared empty (bands of DNA were not visible). | ||
| + | * transformations of Pbad was conducted.. The following day, transformations appeared to be contaminated. They looked significantly different than usual, but were still were used to produce plasmid preps. To ensure that E.coli was present gram staining was performed. Staining showed that no E.coli was present in the cultures. Minipreps and Nanodrop were used to quantify the DNA from the plasmid preps. The results from the Miniprep/Nanodrop varied (some plasmid preps had a decent DNA concentration, others didn’t). Even though yields were not great the samples were run on a gel. However no bands were visible. | ||
Revision as of 14:36, 13 July 2011
6/06/2011-6/10/2011
- included a boot camp.
- different topics were discussed (i.e. biobricks, gene regulation, differences between eukaryotes and prokaryotes).
- boot camp also included a computer science component (focused on Clotho).
- miniprepes were done on previously transformed BFP2. After minipreps, the Nanodrop was used to quantify the DNA. Minipreps did not have high DNA concentration, but were run on a gel (to show presence of DNA). Most lanes appeared empty (bands of DNA were not visible).
- transformations of Pbad was conducted.. The following day, transformations appeared to be contaminated. They looked significantly different than usual, but were still were used to produce plasmid preps. To ensure that E.coli was present gram staining was performed. Staining showed that no E.coli was present in the cultures. Minipreps and Nanodrop were used to quantify the DNA from the plasmid preps. The results from the Miniprep/Nanodrop varied (some plasmid preps had a decent DNA concentration, others didn’t). Even though yields were not great the samples were run on a gel. However no bands were visible.
