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Team:Freiburg/Notebook/28 June
From 2011.igem.org
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| - | <span style="color:blue;">Comments: PCR did not work, because of too high concentration of primers</span> | + | <span style="color:blue;">Comments: PCR did not work, because of too high concentration of primers. PCR was repeated on june, 30</span> |
==<span style="color:green;">Digestion of minipreps for the green light receptor</span>== | ==<span style="color:green;">Digestion of minipreps for the green light receptor</span>== | ||
Revision as of 13:50, 13 July 2011
Contents |
21. Labday
3A assembly:Digestion, Ligation and Transformation for the receptor
Investigators: Julia
Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).
Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI
Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI
ligated vector was then transformed in cells and plated out on chloramphenicol plates.
PCR of GFP fused to plastic-binding domain
Investigators: Sophie, Rüdiger
S 14: GFP
Primer:
- GFPxb1Iup (P1)
- GFPpbdSpedw (P3)
Comments: PCR did not work, because of too high concentration of primers. PCR was repeated on june, 30
Digestion of minipreps for the green light receptor
Investigators: Sophie
Digestion of minipreps:
- S 17 (Ccar 1:1)
- S 18 (Ccar 1:3)
- S 19 (Ccas 1:1)
- S 20 (Ccas 1:3)
- S 21 (artificial terminator)
Enzyme 1: XbaI Enzyme 2: PstI
two gels were loaded.
Comments: S17, S18 and S20 were ok. S21 was not digested.
