Team:Freiburg/Notebook/28 June

From 2011.igem.org

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two gels were loaded.
two gels were loaded.
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<span style="color:blue;">Comments:*S17, S18 and S20 were ok.*S21 was not digested.</span>
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<span style="color:blue;">Comments:S17, S18 and S20 were ok. S21 was not digested.</span>

Revision as of 13:47, 13 July 2011

Contents

21. Labday

3A assembly:Digestion, Ligation and Transformation for the receptor

Investigators: Julia

Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).

Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI

Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI

ligated vector was then transformed in cells and plated out on chloramphenicol plates.


PCR of GFP fused to plastic-binding domain

Investigators: Sophie, Rüdiger


S 14: GFP

Primer:

  • GFPxb1Iup (P1)
  • GFPpbdSpedw (P3)

Comments: PCR did not work, because of too high concentration of primers

Digestion of minipreps for the green light receptor

Investigators: Sophie

Digestion of minipreps:

  • S 17 (Ccar 1:1)
  • S 18 (Ccar 1:3)
  • S 19 (Ccas 1:1)
  • S 20 (Ccas 1:3)
  • S 21 (artificial terminator)

Enzyme 1: XbaI Enzyme 2: PstI

two gels were loaded.

Comments:S17, S18 and S20 were ok. S21 was not digested.

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